| Tuesday 15 September 2009 | |||
| Atletico Madrid | 0-0 | Apoel Nicosia | 19:45 |
| Wolfsburg | 3-1 | CSKA Moscow | 19:45 |
| Chelsea | 1-0 | Porto | 19:45 |
| Maccabi Haifa | 0-3 | Bayern Munich | 19:45 |
| Marseille | 1-2 | Milan | 19:45 |
| Juventus | 1-1 | Bordeaux | 19:45 |
| Zurich | 2-5 | Real Madrid | 19:45 |
| Besiktas | 0-1 | Manchester United | 19:45 |
| Wednesday 16 September 2009 | |||
| Liverpool | 1-0 | Debrecen | 19:45 |
| Lyon | 0-0 | Fiorentina | 19:45 |
| Sevilla | 2-0 | Unirea Urziceni | 19:45 |
| Dynamo Kiev | 3-1 | Rubin Kazan | 19:45 |
| Inter | 0-0 | Barcelona | 19:45 |
| Stuttgart | 1-1 | Rangers | 19:45 |
| Olympiakos | 1-0 | AZ Alkmaar | 19:45 |
| Standard Liege | 2-3 | Arsenal | 19:45 |
| Tuesday 29 September 2009 | |||
| Rubin Kazan | 1-1 | Inter | 17:30 |
| Arsenal | 2-0 | Olympiakos | 19:45 |
| Rangers | 1-4 | Sevilla | 19:45 |
| AZ Alkmaar | 1-1 | Standard Liege | 19:45 |
| Fiorentina | 2-0 | Liverpool | 19:45 |
| Debrecen | 0-4 | Lyon | 19:45 |
| Unirea Urziceni | 1-1 | Stuttgart | 19:45 |
| Barcelona | 2-0 | Dynamo Kyiv | 19:45 |
| Wednesday 30 September 2009 | |||
| CSKA Moscow | 2-1 | Besiktas | 17:30 |
| Milan | 0-1 | Zurich | 19:45 |
| Bordeaux | 1-0 | Maccabi Haifa | 19:45 |
| Bayern Munich | 0-0 | Juventus | 19:45 |
| Porto | 2-0 | Atletico Madrid | 19:45 |
| Real Madrid | 3-0 | Marseille | 19:45 |
| Apoel Nicosia | 0-1 | Chelsea | 19:45 |
| Manchester United | 2-1 | Wolfsburg | 19:45 |
| Tuesday 20 October 2009 | |||
| Debrecen | 3-4 | Fiorentina | 19:45 |
| Rangers | 1-4 | Unirea Urziceni | 19:45 |
| Inter | 2-2 | Dynamo Kyiv | 19:45 |
| Liverpool | 1-2 | Lyon | 19:45 |
| Stuttgart | 1-3 | Sevilla | 19:45 |
| AZ Alkmaar | 1-1 | Arsenal | 19:45 |
| Barcelona | 1-2 | Rubin Kazan | 19:45 |
| Olympiakos | 2-1 | Standard Liege | 19:45 |
| Wednesday 21 October 2009 | |||
| CSKA Moscow | 0-1 | Manchester United | 17:30 |
| Porto | 2-1 | Apoel Nicosia | 19:45 |
| Zurich | 0-1 | Marseille | 19:45 |
| Real Madrid | 2-3 | Milan | 19:45 |
| Juventus | 1-0 | Maccabi Haifa | 19:45 |
| Wolfsburg | 0-0 | Besiktas | 19:45 |
| Chelsea | 4-0 | Atletico Madrid | 19:45 |
| Bordeaux | 2-1 | Bayern Munich | 19:45 |
| Tuesday 3 November 2009 | |||
| Marseille | 6-1 | Zurich | 19:45 |
| Bayern Munich | 0-2 | Bordeaux | 19:45 |
| Maccabi Haifa | 0-1 | Juventus | 19:45 |
| Manchester United | 3-3 | CSKA Moscow | 19:45 |
| Apoel Nicosia | 0-1 | Porto | 19:45 |
| Besiktas | 0-3 | Wolfsburg | 19:45 |
| Atletico Madrid | 2-2 | Chelsea | 19:45 |
| Milan | 1-1 | Real Madrid | 19:45 |
| Wednesday 4 November 2009 | |||
| Rubin Kazan | 0-0 | Barcelona | 17:30 |
| Dynamo Kyiv | 1-2 | Inter | 19:45 |
| Lyon | 1-1 | Liverpool | 19:45 |
| Sevilla | 1-1 | Stuttgart | 19:45 |
| Standard Liege | 2-0 | Olympiakos | 19:45 |
| Fiorentina | 5-2 | Debrecen | 19:45 |
| Arsenal | 4-1 | AZ Alkmaar | 19:45 |
| Unirea Urziceni | 1-1 | Rangers | 19:45 |
| Tuesday 24 November 2009 | |||
| Rubin Kazan | 0-0 | Dynamo Kyiv | 17:30 |
| Unirea Urziceni | 1-0 | Sevilla | 19:45 |
| Rangers | 0-2 | Stuttgart | 19:45 |
| Barcelona | 2-0 | Inter | 19:45 |
| Debrecen | 0-1 | Liverpool | 19:45 |
| Arsenal | 2-0 | Standard | 19:45 |
| AZ Alkmaar | 0-0 | Olympiakos | 19:45 |
| Fiorentina | 1-0 | Lyon | 19:45 |
| Wednesday 25 November 2009 | |||
| CSKA Moscow | 2-1 | Wolfsburg | 17:30 |
| Bayern Munich | 1-0 | Maccabi Haifa | 19:45 |
| Manchester United | 0-1 | Besiktas | 19:45 |
| Real Madrid | 1-0 | Zurich | 19:45 |
| Apoel Nicosia | 1-1 | Atletico Madrid | 19:45 |
| Porto | 0-1 | Chelsea | 19:45 |
| Milan | 1-1 | Marseille | 19:45 |
| Bordeaux | 2-0 | Juventus | 19:45 |
| Tuesday 8 December 2009 | |||
| Atletico Madrid | 0-3 | Porto | 19:45 |
| Wolfsburg | 1-3 | Manchester United | 19:45 |
| Maccabi Haifa | 0-1 | Bordeaux | 19:45 |
| Zurich | 1-1 | Milan | 19:45 |
| Besiktas | 1-2 | CSKA Moscow | 19:45 |
| Marseille | 1-3 | Real Madrid | 19:45 |
| Juventus | 1-4 | Bayern Munich | 19:45 |
| Chelsea | 2-2 | Apoel Nicosia | 19:45 |
| Wednesday 9 December 2009 | |||
| Dynamo Kyiv | 1-2 | Barcelona | 19:45 |
| Inter | 2-0 | Ruib Kazan | 19:45 |
| Stuttgart | 3-2 | Unirea Urziceni | 19:45 |
| Sevilla | 1-0 | Rangers | 19:45 |
| Lyon | 4-0 | Debrecen | 19:45 |
| Olympiakos | 1-0 | Arsenal | 19:45 |
| Liverpool | 1-2 | Fiorentina | 19:45 |
| Standard Liege | 1-1 | AZ Alkmaar | 19:45 |
| Tuesday 16 February 2010 | |||
| Milan | 2-3 | Manchester United | 19:45 |
| Lyon | 1-0 | Real Madrid | 19:45 |
| Wednesday 17 February 2010 | |||
| Bayern Munich | 2-1 | Fiorentina | 19:45 |
| Porto | 2-1 | Arsenal | 19:45 |
| Tuesday 23 February 2010 | |||
| Olympiakos | 0-1 | Bordeaux | 19:45 |
| Stuttgart | 1-1 | Barcelona | 19:45 |
| Wednesday 24 February 2010 | |||
| CSKA Moscow | 1-1 | Sevilla | 17:30 |
| Inter | 2-1 | Chelsea | 19:45 |
| Tuesday 9 March 2010 | |||
| Arsenal | 5-0 | Porto | 19:45 |
| Fiorentina | 3-2 | Bayern Munich * | 19:45 |
| Wednesday 10 March 2010 | |||
| Manchester United | 4-0 | Milan | 19:45 |
| Real Madrid | 1-1 | Lyon | 19:45 |
| Tuesday 16 March 2010 | |||
| Chelsea | 0-1 | Inter | 19:45 |
| Sevilla | 1-2 | CSKA Moscow | 19:45 |
| Wednesday 17 March 2010 | |||
| Barcelona | 4-0 | Stuttgart | 19:45 |
| Bordeaux | 2-1 | Olympiakos | 19:45 |
| Tuesday 30 March 2010 | |||
| Bayern Munich | 2-1 | Manchester United | 19:45 |
| Lyon | 3-1 | Bordeaux | 19:45 |
| Wednesday 31 March 2010 | |||
| Arsenal | 2-2 | Barcelona | 19:45 |
| Inter | 1-0 | CSKA Moscow | 19:45 |
| Tuesday 6 April 2010 | |||
| CSKA Moscow | 0-1 | Inter | 17:30 |
| Barcelona | 4-1 | Arsenal | 19:45 |
| Wednesday 7 April 2010 | |||
| Bordeaux | 1-0 | Lyon | 19:45 |
| Manchester United | 3-2 | Bayern Munich * | 19:45 |
| Tuesday 20 April 2010 | |||
| Inter | 3-1 | Barcelona | 19:45 |
| Wednesday 21 April 2010 | |||
| Bayern Munich | 1-0 | Lyon | 19:45 |
| Tuesday 27 April 2010 | |||
| Lyon | 0-3 | Bayern Munich | 19:45 |
| Wednesday 28 April 2010 | |||
| Barcelona | 1-0 | Inter | 19:45 |
| Saturday 22 May 2010 | |||
| Inter | 2-0 | Bayern Munich | 19:45 |
| * = win on away goals | |||